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KMID : 1004820120130020157
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Journal of Biomedical Research
2012 Volume.13 No. 2 p.157 ~ p.164
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Inhibitory Effect of Natural Killer Cells on Liver Tumor Growth in Mouse Xenograft Model
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Park Ji-Sung
Sung Hye-Ran
Kim Il-Hoi
Lee Chong-Kil
Hong Jin-Tae
Song Suk-Gil
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Abstract
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Human natural killer (NK) cells are major players in innate immune response. The functions of these cells as a scavenger of cancer cells are enhanced by cytokines such as interleukin-2 (IL-2), which play an important role in immune response in both tumors and virally infected cells. Liver cancer has a high incidence rate and is a major cause of death in Korea. We provide evidence that human NK cells inhibit tumor growth of the hepatocellular carcinoma cell line SNU-354. NK cells were cultured with human IL-2 for 14 days, yielding an enriched NK cell population containing 35% CD8+cells, 6% CD4+cells, and 51% CD16+/CD56+ cells. Intravenous injection of NK cells at doses from 2.5 to 10 million cells/mouse was administered once per week in a nude mouse model that retains human liver tumor induced by implantation of SNU-354 cells. The results showed that human NK cells were recruited within tumor tissue and inhibited SNU-354 tumor growth by 32%, 58%, and 65%. The current data suggest the potential for use of NK cell-based immunotherapy for treatment of human liver cancer.
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KEYWORD
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Natural killer cells, SNU354 hepatoma cell line, Immunotherapy
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